Separation of rabbit marrow precursor cells by combined isopycnic sedimentation and electronic cell sorting

Separation of rabbit marrow precursor cells by combined isopycnic sedimentation and electronic cell sorting.

Separation of developing cells into fractions of differing stages of maturity is critical to effective biochemical study of the process of cellular differentiation. Density gradient techniques utilizing rate-zonal or isopycnic separations have permitted partial separations based on cell mass or cell density. In this study the separation of various rabbit marrow cells was improved by high-speed ...

Separation of rabbit adrenal cells by sedimentation at a linear gradient of albumin concentration.

The isolated cells of rabbit adrenal glands were separated by the method of sedimentation at a linear gradient of albumin concentration and incubated with 14C-cholesterol. The corticosterone and cortisol produced by the separated cells were fractionated by thin-layer chromatography and the radioactivities in these steroids were measured. The more rapidly sedimenting cells showed a greater ratio...

Separation of subcellular components and viruses by combined rate- and isopycnic-zonal centrifugation.

The re-isolation of trace anlOunts of virus added to tissue hOInogenates has been taken as a Inodel probleIll in the developIllent of high-resolution Illethods for separating particles. FroIll a review of data on these particles, it was found that, when sediIllentation rates are plotted against their banding or buoyant densities, IllOst viI'uses fall in a "window" which appears to be free of ot...

Separation of erythroid progenitor cells in mouse bone marrow by isokinetic-gradient sedimentation.

Isokinetic-gradient sedimentation employing a shallow linear gradient of Ficoll in tissue culture medium was used to isolate erythroid progenitor cells (CFU-e) from mouse bone marrow. Following gradient sedimentation, 34% of the total nucleated cells and 48% of the CFU-e applied to the gradient were recovered, and three distinct modal populations of CFU-e could be distinguished. The slowest-mig...

Separation of immunocompetent cells from human and mouse hemopoietic cell suspensions by velocity sedimentation.